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In this paper, we investigate on the use of Surface Plasmon to provide a powerful detect of a wide diversity of bimolecular interactions. The advantage of the nanobiosensors based on Surface Plasmon Resonance is to monitor molecular interactions in real times and label-free. These nanobiosensors provide a means for identifying these interactions and quantifying their kinetic constants, equilibrium constants and underlying energetic. This identification is released in our work by the study of the dispersion characteristics and the reflectivity of Surface Plasmon waves by using the Transverse Resonance Method.